Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Boca Scientific Inc Antibody Stabilizer PBS Base, 125ml
Antibody Stabilizer conserves the structure of proteins and antibodies to prevent loss of functionality due to storage. With refrigerated storage, Antibody Stabilizer prevents the need for freezing and thawing. Both TRIS-based and PBS-based Antibody Stabilizers are available, allowing the user to select the optimal buffer base for the assay.
Bioworld Carbonate-buffered saline pH 9.5 (5X), 500 mL
This buffer contains 125mM sodium carbonate and 750mM sodium chloride, pH 9.5. The buffer is important for several different cellular processes. It is one of the major buffering systems used to maintain the pH of mammalian blood and is also important in the acid-base homeostasis of living things, including humans. It tends to maintain a relatively constant plasma pH and counteract any force that would alter it.Warning:For laboratory use only.Not fit for agricultural, household, clinical use or humanconsumption. Appearance: LiquidPH Value: 9.5
Growcells PBS 1X 1L EA
Ready to use, sterile 1X Phosphate-Buffered Saline solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 8g, KCl 0.2g, Na2HPO4 1.44g, KH2PO4 0.24g
Aniara Diagnostica LLC PBS PDR PH 7.4 10L
Medicago Phosphate Buffered Saline (PBS) pH 7.4 supplied as preweighed powder mix in sealed pouch yields 10 L 0.14 M NaCl, 0.0027 M KCl, 0.010 M phosphate buffer with pH 7.4 at 25C. Used as biological buffer, isotonic. 1 pouch.
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 100ML
Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 100ML
AdipoGen PBS Endotoxin-free (sterile), 10 mL
Synonyms: Phosphate Buffer Saline Endotoxin-free (sterile). Endotoxin free buffer used for the dilution of TLRpure LPS and solubilisation of endotoxin-free reagents (e.g. ODNs).
Teknova 1X PBS with 0.05% Tween 20, 1% BSA, 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Sigma Aldrich Fine Chemicals Biosciences TrizmaR hydrochloride solu
A series of Pre-mixed SOLUTIONons of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy /- 0.1 pH units.
Kd Medical Inc PBS 20X Solution, PH 7.4, 1L
Phosphate Buffered Saline in a 20X concentrate for use as a wash buffer in immuno-labeling techniques. pH 7.4 One 1 Liter bottle.
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Crude cell extracts contain a variety of endogenous enzymes capable of degrading proteins such as phosphatases and proteases The optimal method to enhance the yield of intact proteins is to supplement with inhibitors targeting these known enzymes This Protease Inhibitor Cocktail is used in plant cell and tissue extracts to improve protein stability The cocktail inhibits proteases that degrade both non-phosphorylated and phosphorylated protein substrates The Protease Inhibitor Cocktail contains individual components including AEBSF 1 10-Phenanthroline Bestatin E-64 Leupeptin and Pepstatin A providing broad specificity against cysteine serine aspartic metalloproteases and aminopeptidases This protease inhibitor cocktail has been optimized and tested specifically for plant cell and tissue extracts Provided as a ready-to-use solution this protease inhibitor cocktail is supplied in DMSO as the solvent
![Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 98.6g make, 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661308.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 98.6g make, 1 L
PBS [Phosphate Buffered Saline] 10X Powder Concentrate is a white granular powder that, when dissolved in water, creates a 10X concentrated PBS solution. This 98.6g pack makes 1 liter of 10X PBS, providing a convenient and efficient way to prepare large volumes of phosphate-buffered saline. The powder dissolves quickly and is ideal for laboratories that need a concentrated PBS solution for dilutions or experiments that require higher concentration buffers. PBS is widely used in cell culture, molecular biology, and protein studies, and the 10X concentrate is ideal for reducing storage space and simplifying buffer preparation in the lab.
Sigma Aldrich Fine Chemicals Biosciences HEPES potassium salt | 82207-62-3 | MFCD00043438 | 25 g
HEPES potassium salt | Purity: ≥99.5% | Mol Wt: 276.39 | 82207-62-3 | MFCD00043438 | 25 g